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After 48 h the cells were washed then fixed and permeabilized with cold 70% ethanol for 2 h at -20C then washed with cold PBS twice

Siger, A., Nogala-Kalucka, M

6.2.3 Demyelinating injury 6.2.3.1 Myelination disorder Human myelination begins in mid-gestation and peaks during the first few years after birth, continuing into adulthood (Purger et al., 2016)

Consequently, by inhibiting the cascade amplification of peripheral inflammatory signals to the central nervous system, it indirectly alleviated central nervous system inflammation and exerted a neuroprotective effect ( 4 Conclusions and outlook Treatment for PD must address both motor and non-motor symptoms

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